IDEXX SimPlate for HPC

IDEXXSKU: 98-05760-01
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Description

SimPlate for HPC

Heterotrophic plate counts, simplified


                    

Easy-to-count wells on a round plate that correlates to plate count agar. Positives fluoresce under UV light.

Overview

Easy
  • Easier to read and use than the pour-plate method.
  • No agar preparation.
  • Most Probable Number (MPN)  counting range minimises need for dilutions.
Rapid
  • Instant media preparation.
  • Less than 2 minutes of hands-on time.
  • Reduced read time.
Accurate
  • Simplified procedure minimises chance of contamination.
  • Less subjective interpretation than pour plate

Economical 

  • Less equipment to purchase and maintain.
  • Increased productivity saves labour costs.
  • Minimal Quality Control (QC).

Science


How SimPlate for HPC works

Multiple Enzyme Technology Medium—the SimPlate for HPC medium uses IDEXX Multiple Enzyme Technology (MET) to detect heterotrophic plate count (HPC) bacteria in water. The reagent contains multiple unique enzyme substrates, each targeting a different bacterial enzyme. The most common enzymes of waterborne bacteria are all targeted.

All of the enzyme substrates produce the same signal when metabolised. Therefore, a given waterborne bacteria will be able to metabolise one or more enzyme substrates and produce a signal. In the case of SimPlate for HPC, the signal is fluorescence under 365 nm UV light.

After the sample and medium are added to the plate, the wells fill automatically as the user swirls the plate. No pipetting is needed.

Once the wells are filled, the plate is angled and excess sample/medium mixture is poured into the absorbent pad. The SimPlate takes advantage of surface tension to hold the proper amount of sample/medium mixture in each of its 84 wells as excess is poured off.

SimPlates are incubated upside down to prevent condensation from collecting on the lid. Since each well is isolated from the others, bacteria from one well will cause only that one well to fluoresce.


The number of wells that fluoresce is converted to a Most Probable Number (MPN) using the table provided with the product. The high number of wells (84) results in a very high counting range and precise 95% confidence limits. The counting range is from <1 to 738 per plate (more than double a standard pour plate) with the multi-dose SimPlate for HPC.
 

 

Instructions


Step 1

Add 100 mL sterile diluent to media vessel.


Step 2

Add 1 mL sample to SimPlate.


Step 3

Add 9 mL media to SimPlate.


Step 4

Swirl SimPlate and pour off excess.


Step 5

Invert and incubate for 48 hours.


Step 6

Read results under UV light.

 

Product Information

SimPlate for HPC, unit-dose kit (25 tests)
Product number: 98-05760-01
Catalogue number: WHPC-25

SimPlate for HPC, multi-dose kit (100 tests, 10 media vessels and 100 SimPlates)
Product number: 98-05761-01
Catalogue number: WHPC-100

SimPlate for HPC, media-only kit (40 tests)
Product number: 98-12860-00
Catalogue number: WHPC-40M

IDEXX-QC HPC/TVC Kit
Product Number: 98-29006-01
Catalogue Number: WQC-HPC

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